Autoclave manufacturer in delhi

Posted by Lifeclave on February 4th, 2021

Autoclave Use

Autoclaves provide a physical method for disinfection and sterilization. They work with a combination of steam, pressure and time. Autoclaves operate at high temperature and pressure in order to kill microorganisms and spores.

They are used to decontaminate certain biological waste and sterilize media, instruments and lab ware. Regulated medical waste that might contain bacteria, viruses and other biological material are recommended to be inactivated by autoclaving before disposal. Lifeclave is the best autoclave manufacturers in delhi.

Autoclave Cycles

  • To be effective, the autoclave must reach and maintain a temperature of 121° C for at least 30 minutes by using saturated steam under at least 15 psi of pressure. Increased cycle time may be necessary depending upon the make-up and volume of the load.
  • The rate of exhaust will depend upon the nature of the load. Dry material can be treated in a fast exhaust cycle, while liquids and biological waste require slow exhaust to prevent boiling over of super-heated liquids. 

Material  

Recommended for:

 
 

Liquids cycle

(Slow Exhaust)

Use with glass containers with vented closures; 2/3 full only

•Liquid media

•Nonflammable liquids

•Aqueous solutions

•Liquid biological waste

 

Solids or Dry cycle

(Fast Exhaust)

  • Glassware: empty and inverted
  • no tight or impermeable closures
  • Dry hard items, either unwrapped or in porous wrap
  • Metal items with porous parts
  • Other porous materials
 

Wrapped Goods or Pre vacuum cycle

(Clean: Fast Exhaust

Dirty: Slow Exhaust)

  • Glassware that must be sterilized upright and/or can trap air
  • Wrapped dry items that can trap air
  • Pipette tip boxes
  • Sharps decontamination
  • (in collection containers)
  • Biohazard waste decontamination, in autoclave bags; can be wet or dry

Like it? Share it!


Lifeclave

About the Author

Lifeclave
Joined: February 4th, 2021
Articles Posted: 2

More by this author