FACTS TO KNOW ABOUT ADENOVIRUS VECTOR SYSTEM AND ITS PRODUCTION

Posted by Heiwhite on October 7th, 2019

The Adenoviridae family is very versatile and it consists of different types of adenovirus constituents. In 1953, the human adenoids were isolated initially from which the Adenovirus name is derived. The adenovirus vector is not small in the size and it is generally considered in the category of medium-sized virus ranging between 90nm to 100nm. The adenovirus vector consists of the virus which is not enveloped. It also has the icosahedral nucleocapsid which is very crucial in the DNA genome as it is having the genome of double-stranded type. The virus has numerous numbers of vertebrate hosts. It is responsible for the isolation of serotypes which is calculated to be 100 and in humans, the count of an adenoviral serotype is increased to 57 which causes numerous types of diseases. This affects the respiratory system of children by infecting it mildly and in adults, it increases its effects in multiple organs, spreading the disease that is proven to be life-threatening. The molecular biology of mammals can be investigated due to the isolation of adenovirus and it is considered to be an invaluable tool. Let's take a look at some more facts of the adenovirus vector system and further its production and transduction.

Why Adenovirus Gene Therapy?

In 1965, Robert Atchison carried research with his partners in which they get stumbled at a position where they crossed their way with the adeno-associated virus (AAV). The research was carried out in the laboratory of the University of Pittsburgh. In the initial findings, they tend to suppose that the AAV was just the by-product of their research of adenovirus. They carried the research further to find out all the outcomes and the value of this by-product and how it occurred. The particles of AAV are not able to get replicated by themselves within the host cells, but they can do the same and replicate themselves if the cells also get infected by the adenovirus. The adenovirus vector system is widely used in gene therapy and you can find more information on the same on the website https://www.genemedi.net/i/adenovirus-packaging. The Adenovirus gene therapy serves some major advantages, but one very important part of using AAV is that the adenovirus transduction to the genetic materials in the cells of the patients is very efficient and does not tend to cause any harm or illness to the patients. It also doesn’t reproduce itself because it is a sort of dependovirus that further needs to get infected to replicate itself.

Production of Adenovirus

The production of the adenovirus is divided into various phases which extends from the construction of plasmid to the applications of titers. The adenovirus vector is constructed by various methods. The construction consists of two plasmids in which one is the shuttle vector and the second one is the content that is required as the adenovirus genes to start the production of the adenovirus. The first phase takes about one week and then the stage of the initial production gets started. Adenovirus production in the second phase tends to take at least two to three weeks. The whole phase is ended by various phases of titers and in between the process of amplification and purification takes place. After all the phases are done the adenovirus gets ready to be used in further experiments.