Overlooked Advantages of Polyclonal Antibodies

Posted by eliteinternet on December 15th, 2020

Their multi-epitope specificity mainly determines the advantages of using polyclonal antibodies. This post will discuss the key advantages of polyclonal antibodies and why you should consider them a wise investment.

The benefits of polyclonal antibodies as a tool for assay-specific target discovery and detection are just countless. The future of basic diagnostics, research, and biomarker discovery are dependent on superior quality reproducible data. There is a need to understand the benefits and importance of these valuable tools, including polyclonal antibodies like rabbit antibody production services.

Polyclonal antibodies are more sensitive in certain assays against various target cells, organisms, or proteins. The biophysical diversity of polyclonal antibodies allows for greater stability when environmental challenges may cause lability, inactivation, or precipitation of other forms of antibodies. These two properties are the foundation of numerous advantages polyclonal antibodies offer. Before going further, let’s learn some of their key benefits below.

Low costand short production time. Highly stable and tolerant of pH or buffer changes. Since these antibodies bind to more than one epitope, they can help deepen the target protein signal even with a low expression level. That makes polyclonal antibodies ideal for immunoprecipitation and chromatin immunoprecipitation. Tolerant of small changes of antigens. Polyclonal antibodies are less sensitive to antigen changes like polymorphism, slight denaturation, and glycosylation heterogeneity, unlike monoclonal antibodies.

In the research environment, multi-epitope binding properties for polyclonal antibodies have clear benefits. The heterogeneous binding of distinct epitopes and antigens by polyclonal antibodies renders these reagents more likely to bind to a particular antigen in variousimmunoassays and test conditions. That means polyclonal antibodies like GCN2 Kinaseare more appropriate for use than their counterparts.

Polyclonal antibodies also offer higher sensitivity ranges due to their more effective capturing of multiple antigen variants or epitopes presented by the analytes. Because polyclonal antibodies like LIMK1typically recognize multiple epitopes on a target protein, they are more effective at diagnosing a target for use in chromatin immunoprecipitation. In immunohistochemistry (IHC), where the effects of the tissue processing and fixation on the epitope are unknown and highly variable, polyclonal antibodies can be a better option because their multi-epitope binding allows for antigen identification. Furthermore, polyclonal antibodies offer greater sensitivity for identifying proteins present in low quantities in a sample. After knowing these benefits, if you want polyclonal antibodies for your specific project, rely on a reputed polyclonal antibody production facility.