A Brand-new Long-term Neglected Protein Switch FoundPosted by Jerry Carter on June 1st, 2021 Proteins play a wide range of functions in the cells of every organism and play a key role in almost every biological process. They are not only responsible for our metabolism, manage cell signaling, and are responsible for energy production, as antibodies, they are also frontline workers of our immune system against human pathogens such as coronaviruses. Given these important responsibilities, it is not surprising that the activity of proteins is tightly controlled. Published in Nature, the researchers investigated a protein (transaldolase) from the human pathogen Neisseria gonorhoae, which causes gonorrhea, a bacterial infection that has more than 100 million cases worldwide. This disease is usually treated with antibiotics, but the increasing rate of antibiotic resistance poses a serious threat. To identify new treatments, they studied the structure and mechanism of a protein that plays a key role in carbon metabolism in pathogens. Surprisingly, the protein can be switched on and off by oxidation and reduction (called a "redox switch"). Scientists suspect that this is caused by a common, well-established "disulfide switch" formed between the two cysteine amino acids. When they deciphered the X-ray structure of this protein in the “on” and “off” states at the DESY particle accelerator in Hamburg, Germany, they harvested greater surprise. The chemical nature of this switch is completely unknown: it is formed between lysine and cysteine amino acids with a bridging oxygen atom. “I can't believe my eyes,” said Professor Kai Tittmann, who led the study, recalling the first time he saw the structure of this novel switch. “We initially thought this must be a by-product of the experimental process because this chemical entity is unknown. But multiple repeated experiments always get the same results, and analysis of protein structure databases further reveals that there are many other proteins that are likely to have this switch, which clearly escapes early detection because protein structure analysis is not sufficiently resolved to detect it.” The researchers acknowledge that luck is on their side because the crystals they measure are able to determine protein structure with extremely high resolution, which leads to this novel switch that cannot eventually be missed. Like it? Share it!More by this author |