The cyanide series dyes

Posted by beauty33 on October 28th, 2021

The earliest application of dyes in biochemistry was to dye slices directly and then observe them. With the continuous development of biotechnology, computer technology and fluorescence spectrometry technology, many dyes, especially fluorescent dyes, have been widely used in cell detection, tumor gene protein analysis, toxicant analysis, and clinical medical diagnosis. Fluorescent dyes generally refer to substances that absorb light waves of a certain wavelength and emit light waves larger than the wavelength of the absorbed light. The use of fluorescent dyes for antibody labeling analysis is widely used in the field of modern biological immunology, and has gradually shown obvious advantages.

The following briefly introduces the fluorescent dyes and their types used to label antibodies:

1. Fluorescein dyes, including fluorescein isothiocyanate (FITC), hydroxyfluorescein (FAM), tetrachlorofluorescein (TET), etc. and similar Things. This is a class of compounds with more benzene rings. The most widely used is FITC (figure shows the FITC-labeled tissue fluorescence image), which is excited by an argon ion laser at 488nm and emits blue-green fluorescence at 525nm. FITC can bind to various antibody proteins and exhibit blue-green fluorescence stably in alkaline solution.

2. Rhodamine dyes, including red rhodamine (RBITC), tetramethylrhodamine (TAMRA), rhodamine B (TRITC), etc. TRITC can emit yellow fluorescence at 570 nm when excited at 550 nm.

3. Cy series cyanine dyes. Cyanine dyes usually consist of two heterocyclic systems, including Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, Cy7 and their analogs.

4. Alexa series dyes, which are a series of fluorescent dyes developed by Molecular Probes. Its excitation light and emission light spectrum cover most of the visible light and part of the infrared spectrum, and it is widely used. Its main features are high brightness, stability, instrument compatibility, multiple colors, insensitivity to pH and water solubility. Including Alexa Fluor 350, 405, 430, 488, 532, 546, 555, 568, 594, 610, 633, 647, 680, 700, 750. Currently, Alexa series dyes are widely used in the market, and they are gradually replacing traditional fluorescent dyes. For example, Alexa Fluor 488 can replace FITC and Cy2; Alexa Fluor 555 can replace Cy3 and TAMRA; Alexa Fluor 633 can replace APC, Cy5, etc.

5. Protein dyes, including phycoerythrin (PE), phycocyanin (PC), allophycocyanin (APC), polydin-chlorophyll protein (preCP), etc. Most of them are proteins found in cyanobacteria. Such fluorescent dyes can be coupled with Cy series cyanine dyes to form composite dyes for antibody labeling. For example, PE-Cy3/Cy5/Cy7, APC-Cy7, PerCP-Cy5.5 and so on are commonly used in the market. For scientific researchers, how to choose the right fluorescent dye?

Ideal fluorescent dyes generally have the following characteristics: 1. High photon yield and high signal intensity; 2. Strong absorption of excitation light and lower background signal; 3. Large distance between excitation spectrum and emission spectrum , Reduce the interference of background signals; 4. It is easy to bind to the labeled antigen, antibody or other biological substances without affecting the specificity of the labeled substance; 5. It has good stability and is not susceptible to light, temperature, pH, sample anticoagulant and The effect of fixatives.

About us

BOC Sciences is a life science group, with its headquartered in New York, that aims to provide the most comprehensive and professional fluorescent molecules for researchers in the fields of biotechnology, life science, and pharmaceutical research. Our product portfolio comprehensively covers many types of fluorophores, fluorescent probes, and fluorescent dyes, many of which are not available elsewhere. Here are some our products, rox dyesulfo cyanine5 maleimide, tetrazine, tmr azide, etc.

 

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Joined: July 10th, 2017
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