A Brief Guide on Antigen Retrieval Methods
Posted by Immuno Staining on November 12th, 2018
The fact is that formalin-fixed tissue needs an antigen retrieval procedure before immunohistochemical staining can begin. This is because of the formation of methylene bridges that occurs during fixation. It takeovers link proteins and thus mask antigenic sites. There are two methods recommended for Antigen Retrieval - enzymatic or heat mediated. Both of these Antigen Retrieval Methods will assist to break the methylene bridges in order to expose the sites to endow the antibodies to bind.
Moreover, the enzymatic method is considered as a gentler process than heat mediated method. Thus, it is well recommended for sensitive tissues. It should be noted that the enzymatic method takes much time than the heat mediated method. But this method is quite technically demanding. Moreover, in a case, the antigen retrieval step is stated on the antibody datasheet, start someone by trying the heat mediated method. In a case, you will not succeed, then again try with the enzymatic method.
Let’s comprehensively discuss both processes of Antigen Retrieval Method
In this, tissues procedure is done in the best way on amino-propyl-tri-ethoxy-silane coated slides. Moreover, these should be placed in a standard rack for this process.
Requirement of Reagents
For this Antigen Retrieval Method, there will be no use of the domestic microwave. Only a scientific microwave is recommended for this.
Requirement of Reagents
Booster Antibody and Elisa experts is a prominent destination to get more guidance about Antigen Retrieval Methods. To contact Booster Antibody and Elisa experts visit our website: https://immunostaining.info/
About the AuthorImmuno Staining
Joined: November 12th, 2018
Articles Posted: 22
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