Know Everything about Paraffin Embedded Tissue at

Posted by Immuno Staining on February 12th, 2019

Immunostaining is the website that offers you detailed information about IHC Protocol. It is a small part of Boster bio that gives you complete knowledge about IHC Principle and IHC Protocol. This website is especially designed to educate the people about the detailed process of immunostaining and how it works?

Tissue Sectioning at is Slide Pre-Treatment. The pre-treatment procedure is designed to prevent peeling caused by elevated temperature, high pressure, radiation and other factors. Microscopic Slide includes the oil attached on surface, a new slide dipped into cleaning solution for 12 to 24 hours.

The coverslip pre-treatment procedure is very similar to the one for the microscopic slide except that dipping and cleaning should be completed in 2 hours because the coverslip is much thinner. Tissue Section has different types.

Frozen is the most important feature for this type of tissue section which is to keep antigen’s immune-competence completely, especially for the cell surface antigen. Both fresh and fixed tissues can be processed as frozen tissues. However, the tissues must be dried (or primary fixed) and stored at low temperature.

Paraffin Embedded Tissue section is normally sliced by a rotary microtome to give a thickness of 2-7 μm. With proper treatment, the section reveals clear tissue structure and exact antigen location to enable high medical-value pathology researches and retrospective studies. This section type can be stored at 4℃ for long term use.

About is the website that offers you detailed information about IHC protocol. It is a small part of Boster Bio that gives you complete knowledge about IHC principle and IHC protocol. IHC principle is the method of fixation, tissue sectioning, paraffin embedding, inactivation and blocking, antigen retrieval, detection and so on. You will also study complete guide of IHC Sample preparation guide and IHC protocol. To know more about it, visit us at and get complete information. For more details visit our website

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