Antigen Retrieval Methods, Techniques, Protocols - Immunostaining.info

Posted by Immuno Staining on May 9th, 2019

Consistency is one of the most demanding aspects for the execution of successful biospecimen staining. In an endeavour to accelerate your immunostaining of tissue sections and cell climbing parts, Boster Antibody and Elisa Experts have developed and validated their step-by-step IHC/IF/ ICC protocols to cover all aspects of biospecimen research and assay procedures.

The need for antigen retrieval depends on several variables, including the target antigen, the type of tissue, the antibody used, and the process and duration of fixation. Because they identify multiple epitopes, polyclonal antibodies are more probable than monoclonal antibodies to sense a given antigen without the use of antigen retrieval methods.

There are numerous techniques to refurbish the immunoreactivity of an epitope. For partially masked epitopes it might be suitable to first try escalating the primary antibody incubation conditions previous to beginning antigen retrieval. On the other hand, this step will also require additional optimization in terms of proper antibody concentration, incubation time, and temperature.

When looking at antigen retrieval methods, techniques usually fall into two main categories, PIER (protease-induced epitope retrieval) and HIER (heat-induced epitope retrieval). Once optimized, the actual effects of antigen retrieval can be marked. Most formalin-fixed tissues need an antigen retrieval action before immunohistochemical staining.

Boster Antibody and Elisa Experts consider that these IHC protocols should be a of use resource for staining workflow or at least a first-class starting point for additional protocol optimization, if necessary. For each of these protocols, they also provide a summary flow chart by means of the applicable Boster’s reagents to improve your product hunt on their website.

About Boster Bio
Boster Bio is one of the leading Flow Cytometry Technical Resource Centers. It was founded by Steven Xia, histologist, in 1993. Their products are enclosed by the Boster Quality Guarantee and vigorously mounting with new antibodies and ELISA kits. The corporation currently provides hybridoma generation check to product custom monoclonal antibodies. They are proficient in ELISA kits and antibody-antigen liquid phase reactions.

For more information and IHC Antigen Retrieval Optimization Tips, please visit https://immunostaining.info

Contact Address

Immunohistochemical Staining

3942 Valley Ave

Pleasanton, CA 94566, USA

Email:support@bosterbio.com

Phone: (888) 466-3604

Immuno Staining

About the Author

Immuno Staining
Joined: November 12th, 2018
Articles Posted: 22

+ Sales Rebuttals » Overcoming Objections » Closing Techniques
+ Sales Rebuttals Mp3 Audio Training for Handling Objections - Learn to Overcome Objections w/ 20 Sales Rebuttals & Closing Techniques Examples.
Pete's Betfair Methods - Professional Betfair Training System
Visit Pete's Betfair Methods. Professional Training Systems for those looking to gain an advantage in the Betfair trading exchanges.
How To Solve Sudoku Puzzles - Sudoku Video Tutorials - Sudoku Solving Techniques
Learn How to Solve any Sudoku puzzles with amazing high quality Video Tutorials. Obtain Guru status by learning these advanced sudoku solving techniques such as X Wing, XY Wing, XYZ Wing, Jelly Fish, Sword Fish and more.
Guitar Control | 3 Secrets to Killer Guitar Control by Claude Johnson
Choose our Killer Guitar Control Secrets course to learn how to master the basic skills and advanced playing techniques!
Spray Paint Art Tutorials & Techniques - Spray Paint Art Secrets
Learn Spray Paint Art From the Mexican Originators.A huge spray paint art library of over 400 tutorial videos. Want to learn spray paint, contact us now!
Secrets of the BIG Dogs!
Considered the BIBLE of Internet marketing since 2001! Ebook that details the most effective Internet advertising techniques and residual income generation techniques. Updated yearly.