Perfect upgrade on quality,new breakthroughs in Click Chemistry Series ProductPosted by chemicals on June 11th, 2019 1. A heterobifunctional peg bioorthogonal linker with azide (click reaction) and amine reactivity Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT) Chemoselective – azides and BCN groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency. Amine reactivity – modify amine-containing molecules or biomolecules with BCN moiety for subsequent conjugation/labelling with azide counterpart. 2. Copper-free click click reagents is a bioorthogonal reaction developed as an activated variant of an azide alkyne Huisgen cycloaddition. Cu-free click chemistry has been modified to be bioorthogonal by eliminating a cytotoxic copper catalyst, allowing reaction to proceed quickly and without live cell toxicity. Although the reaction produces a regioisomeric mixture of triazoles, the lack of regioselectivity in the reaction is not a major concern for its applications in bioorthogonal chemistry. More regiospecific and less bioorthogonal requirements are best served by the traditional Huisgen cycloaddition, especially given the low yield and synthetic difficulty (compared to the addition of a terminal alkyne) of synthesizing a strained cyclooctyne. The incredible bioorthogonality of the reaction has allowed the Cu-free click reaction to be applied within cultured cells, live zebrafish, and mice. Dibenzylcyclooctyne (DBCO) is widely used in the Cu-free click chemistry. DBCO eliminates the use of cytotoxic copper catalyst, and allows reaction to proceed quickly and without live cell toxicity. Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT) Chemoselective – azides and DBCO groups do not react or interfere with other functional groups found in biological samples but conjugate to one another with high efficiency Primary amine reactivity – modify amine-containing molecules with DBCO for use in advanced crosslinking experiments. Like it? Share it!More by this author |