BGP-15 (CAS 66611-37-8) - LS-H6785
Posted by freemexy on July 4th, 2019
BGP-15 (CAS 66611-37-8) - LS-H6785
The poly(ADP-ribose) polymerases (PARPs) form a family of enzymes with roles in DNA repair and apoptosis. 66611-37-8 is a PARP inhibitor and insulin sensitizer. In perfused heart, it blocks self-ADP-ribosylation of nuclear PARP (Ki = 57 µM) and mono-ADP-ribosylation of GRP78, resulting in decreased levels of reactive oxygen species, lipid peroxidation, and single-strand DNA breaks. BGP-15 acts as a co-inducer of heat shock protein 72, augmenting expression induced by heat shock factor 1 in adipocytes and skeletal muscles and improving insulin sensitivity.
BGP-15 is supplied as a crystalline solid. A stock solution may be made by dissolving the BGP-15 in the solvent of choice. BGP-15 is soluble in the organic solvent DMSO, which should be purged with an inert gas, at a concentration of approximately 0.25 mg/ml. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of BGP-15 can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of BGP-15 in PBS, pH 7.2, is approximately 5 mg/ml. We do not recommend storing the aqueous solution for more than one day.
