What Is The ELISA Test And Its Procedure?

Posted by Antibody Elisa on July 9th, 2019

An enzyme-linked immunosorbent assay is also known as ELISA or EIA. It is a test that is used to deduct along with measuring antibodies in a blood sample. It is performed by medical professionals to determine whether antibodies have certain infectious conditions. Kindly note that antibodies are proteins that a human body produces in regards to deleterious substances called antigens.

ELISA is basically used as a screening tool prior others in-depth tests are ordered. This test is recommended by doctors only when you notice some symptoms or signs of the conditions written-below. In brief, when you indulge in the Elisa Test Procedure, you will able to diagnose the following:

• HIV, which results in AIDS
• Lyme disease
• pernicious anaemia
• rotavirus
• squamous cell carcinoma
• syphilis
• toxoplasmosis
• varicella-zoster virus, which results in chickenpox and shingles
• Zika virus
• Rocky Mountain spotted fever

How does Elisa Test Procedure perform?

To perform the Elisa test, you don’t need to indulge in a complicated manner. It is simple along with straightforward.

For this, you need to take a blood sample. First of all, you need to clean the arms of the patients with an antiseptic. After this, you need to add a band or tourniquet around your arm to establish pressure. This also helps your veins to swell with blood.

Then, insert a needle in one of the patients’ veins to fetch a small sample of blood. Once you get required blood, you must remove the needle and place a small bandage over that area. Moreover, you must guide the patient to maintain pressure at that site for a few minutes to reduce blood flow.

Send the blood sample to a laboratory for analysis where a technician will place the sample to a petri dish. It has the required antigen related to the condition for which, blood is being tested.

During Sandwich Elisa Protocol, if a blood sample contains antibodies to the antigen then the two will bind together. This will examine by adding an enzyme to the petri dish and analyzing how the blood sample and the antigen react.

The result of this testing depends upon what is the contents of the dish change color. Besides, how much amend the enzyme causes endows the technician to examine the presence and volume of the antibody.

If you need to know more about Elisa test procedure or Elisa Troubleshooting, then you must Booster Antibody and Elisa Experts through its official web portal i.e. https://www.antibody-elisa.com/.