Visualizing How HIV Multiplies In Vivo with High ResolutionPosted by Jerry Carter on November 4th, 2019 To treat a disease, one must understand how it arises. In a new study, researchers from Germany, Britain, France, Spain and Australia now use high-resolution imaging to visualize how HIV spreads between living cells at millisecond resolution. Using ultra-high resolution STED fluorescence microscopy, they provide the first direct evidence that the HIV virus builds a lipid environment for its own replication. In response, they created a method to study how this viral replication may be potentially prevented. The relevant findings were published in the October 2, 2019 issue of the journal Science Advances under the title "HIV-1 Gag specifically constraints PI (4, 5) P2 and cholesterol mobility in living cells creating a nanodomain platform for virus assembly". The corresponding authors of the paper are Professor Christian Eggeling of the University of Jena, Germany, and Delphine Muriaux of the Institute of Infectious Diseases, Montpellier, France. Focus on the plasma membrane of the host cell These researchers focused on the gate on the plasma membrane of the host cell through which HIV appears in the host cell after infection. They used the protein Gag, which coordinates the processes involved in HIV maturation, as a marker. "Where this protein accumulates, these critical processes cause the virus to self-release and infect other cells." explains Eggeling. To decipher these processes, the researchers explored the spread that occurs at the site of budding of HIV particles. They found that only certain lipids interact with the HIV virus. Although in principle these lipids are known, for the first time they were able to demonstrate this interaction directly in living infected cells. Attack Point to Block HIV Replication “This provides us with a potential target for the development of antiviral drugs," Eggeling said. “Understanding which molecules are required for HIV virus to be released from host cells and undergo replication is a critical prerequisite for studying how to prevent this viral infection. With our technology, we can now track this directly.” Eggeling and his team now want to develop antibodies that attack these molecules, thereby inhibiting the spread of the HIV virus. Eggeling, describing his research project, said: "We need not only to study these antibodies from a medical perspective, but also to find out how to use their biophysical interactions to enhance their efficacy. To this end, we analyzed biological processes, i.e., cell-molecule interactions, with the help of physical parameters such as diffusion." Like it? Share it!More by this author |