kiko

kiko

CD Genomics is aiming at providing the research community wi
Joined: November 27th, 2018
Articles Posted: 67

Articles

Published 1 Month Ago
Applications of 16S/18S/ITS Amplicon Sequencing
16S/18S/ITS amplicon sequencing has now been a well-established method. Due to its low cost, shorter cycle and low quality requirement of the sample, 16S/18S/ITS amplicon sequencing is widely used in the study of microorganisms.

Published 1 Month Ago
Principles and Workflow of 16S/18S/ITS Amplicon Sequencing
16S/18S/ITS amplification sequencing uses the next/third generation sequencing platform and performs high throughput sequencing of PCR products from specific regions such as 16S rDNA/18S rDNA/ITS/ functional genes. It overcomes the disadvantage of some mi

Published 1 Month Ago
Applications of Microbial Whole Genome Sequencing
Microorganisms widely exist in nature and are closely related to human life and production. They are generally divided into fungi, actinomycetes, bacteria, spirulina, rickettsia, chlamydia, mycoplasma and viruses. Microbial whole genome sequencing is an i

Published 1 Month Ago
Overview of Metatranscriptomic Sequencing: Principles, Workflow, and Applications
Metatranscriptomic sequencing provides direct access to culturable and non-culturable microbial transcriptome information by large-scale, high-throughput sequencing of transcripts from all microbial communities in specific environmental samples. Metatrans

Published 1 Month Ago
Introduction to Shotgun Metagenomics, From Sampling to Data Analysis
Compared to 16S/18S/ITS amplicon sequencing, metagenomics can provide more information about functional potential of microbial communities and whole-genome sequences. The rapid development and substantial cost decrease in high-throughput sequencing have d

Published 1 Month Ago
The Workflow of Viral Metagenomics
The workflow of viral metagenomics includes sample collection, virus-like particle purification, nucleic acid extraction, library preparation, and metagenomics sequencing. It is similar to metagenomics workflow, but they differ in some steps. First, it is

Published 1 Month Ago
Bioinformatics Analysis of 16S rRNA Amplicon Sequencing
This article provides a brief introduction to good practices for the bioinformatics analysis of 16S rRNA sequencing by NGS (next-generation sequencing). The bioinformatics pipeline involves two main stages: the preprocessing of data (quality control) and

Published 1 Month Ago
Applications of Metagenomics in Biotechnology and Health Care
Metagenomics is the culture-independent genomic research of microbial communities. Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental samples.

Published 2 Months Ago
Plasmid Detection and Complete Plasmid DNA Sequencing
Bacterial plasmids are circular or linear double-stranded DNA molecules defined by their capability of autonomous replication in the hosts. They are critical sources for microbial evolution and genome innovation due to their ability to acquire foreign DNA

Published 2 Months Ago
Dual RNA Sequencing: Definition and Principle, Workflow, and Applications
Dual RNA-Seq is the parallel transcriptomic analysis of bacterial pathogens and their eukaryotic host cells, which generally involves a typical workflow including RNA extraction, rRNA depletion, library preparation, sequencing, and data analyses and inter

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