Articles By kiko

Published 2 Years Ago
Bioinformatics Workflow for Whole Genome Sequencing
Whole genome sequencing (WGS) has the capacity to greatly enhance genomic knowledge and understand mysteries of life by utilizing the most advanced genetic sequencing technologies. WGS can be used for variant calling, genome annotation, phylogenetic analy

Published 2 Years Ago
Targeted Region Sequencing in Human Disease Studies and Clinical Care
Since the introduction of Sanger sequencing in 1977, genetic sequencing has been greatly improved with costs simultaneously falling. With the ability to rapidly produce large volumes of sequencing data, next-generation sequencing (NGS) enables researchers

Published 2 Years Ago
Bioinformatics Analysis of Viral Metagenomic Sequence
Viral metagenomics is the study of viruses in environmental and biological samples by utilizing next generation sequencing that generates very large data sets. Viral metagenomics analyzes viral sequences to deduce the impact of viruses on the environment

Published 2 Years Ago
Metagenomics in Biotechnology and Health Care
Microbes are present almost everywhere. They play a vital role in cycling carbon, release important compounds, and may be associated with infectious diseases. Metagenomics is the culture-independent genomic research of microbial communities. Metagenomics

Published 2 Years Ago
Applications of 16S/18S/ITS Amplicon Sequencing
16S/18S/ITS amplicon sequencing has now been a well-established method. Due to its low cost, shorter cycle and low quality requirement of the sample, 16S/18S/ITS amplicon sequencing is widely used in the study of microorganisms.

Published 2 Years Ago
Principles and Workflow of 16S/18S/ITS Amplicon Sequencing
16S/18S/ITS amplification sequencing uses the next/third generation sequencing platform and performs high throughput sequencing of PCR products from specific regions such as 16S rDNA/18S rDNA/ITS/ functional genes. It overcomes the disadvantage of some mi

Published 2 Years Ago
Applications of Microbial Whole Genome Sequencing
Microorganisms widely exist in nature and are closely related to human life and production. They are generally divided into fungi, actinomycetes, bacteria, spirulina, rickettsia, chlamydia, mycoplasma and viruses. Microbial whole genome sequencing is an i

Published 2 Years Ago
Overview of Metatranscriptomic Sequencing: Principles, Workflow, and Applications
Metatranscriptomic sequencing provides direct access to culturable and non-culturable microbial transcriptome information by large-scale, high-throughput sequencing of transcripts from all microbial communities in specific environmental samples. Metatrans

Published 2 Years Ago
Introduction to Shotgun Metagenomics, From Sampling to Data Analysis
Compared to 16S/18S/ITS amplicon sequencing, metagenomics can provide more information about functional potential of microbial communities and whole-genome sequences. The rapid development and substantial cost decrease in high-throughput sequencing have d

Published 2 Years Ago
The Workflow of Viral Metagenomics
The workflow of viral metagenomics includes sample collection, virus-like particle purification, nucleic acid extraction, library preparation, and metagenomics sequencing. It is similar to metagenomics workflow, but they differ in some steps. First, it is

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