Articles By kiko

Published 8 Months Ago
Exploring the Diversity of Microbial Eukaryotes: Insights and Study Techniques
Living organisms are categorized based on various characteristics, and one classification system revolves around their cellular components, structure, and function. Consequently, organisms, including microorganisms, can be classified as either eukaryotes

Published 8 Months Ago
A Comprehensive Guide to De Novo Genome Assembly
Genome assembly stands as a pivotal objective in sequencing endeavors. De novo genome assembly, a prominent strategy in this field, involves reconstructing a novel genome from scratch without relying on any reference genomic data. It is an approach that a

Published 8 Months Ago
An Introduction to Amplicon Sequencing of 16S/18S/ITS Regions
Amplicon sequencing is a targeted approach that focuses on specific regions of DNA or RNA within a given sample. It involves amplifying and sequencing a particular gene or genomic region of interest. In the field of microbial ecology, the 16S rRNA gene is

Published 9 Months Ago
Applications of Targeted Region Sequencing in Human Disease Studies and Clinical Care
Since the introduction of Sanger sequencing in 1977, genetic sequencing has been greatly improved with costs simultaneously falling. With the ability to rapidly produce large volumes of sequencing data, next-generation sequencing (NGS) enables researchers

Published 9 Months Ago
The Methods of Whole Genome Sequencing
The emergence of whole genome sequencing technology is a revolutionary advancement in all areas of life sciences. Whole genome sequencing can detect variants, including single-nucleotide variants, insertions/deletions, copy number changes, and large scale

Published 9 Months Ago
Detecting Fusion Transcripts by RNA Sequencing in Screening Tumors
A fusion gene is a chimeric gene formed by the fusion of partial sequences of two genes, usually due to chromosomal translocations or deletions. These chimeric genes can form abnormal transcripts or proteins in subsequent biological processes, which can l

Published 9 Months Ago
Degradome Sequencing: Introduction, Features, Workflow, and Applications
MicroRNAs (miRNAs) are a class of small endogenous non-coding RNAs of about 22 nt in length, with hairpin structures as precursors, which are widely found in animal, plant and viral organisms. Numerous studies have shown that miRNAs are involved in a wide

Published 9 Months Ago
Introduction to Aerobic and Anaerobic Culture
As mentioned in the previous article, one of the conditions needed to be taken into consideration is the oxygen requirement of the microorganisms. Some common microorganisms such as Escherichia coli and Bacillus sp. are usually cultured in the laboratory

Published 9 Months Ago
Microorganisms Fact Sheet: Definition, Types, and Importance
Microorganisms can be found everywhere, in the coldest or hottest environment, in the air, in water, in soil, in plants, and even inside the human body. These organisms have a big contribution to the ecosystem and to the biosphere as a whole.

Published 9 Months Ago
Blood RNA Sequencing: Introduction, Challenges, Workflow, and Applications
Considering that disease-causing and disease-protecting immune cells need to be transferred from lymphatic and secondary lymphoid organs through the peripheral circulation to their sites of action in disease, a clearer picture of the cells and their actio

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