Articles By kiko

Published 6 Months Ago
16S rRNA Sequencing vs. Metagenome Sequencing: A Guide for Selection
Microbiome research has revolutionized our understanding of complex microbial communities, and two pivotal techniques at the forefront of this exploration are 16S rRNA sequencing and metagenome sequencing. These methodologies, each with unique strengths a

Published 6 Months Ago
Introduction, Challenges, Workflow, and Applications of Blood RNA Sequencing
Considering that disease-causing and disease-protecting immune cells need to be transferred from lymphatic and secondary lymphoid organs through the peripheral circulation to their sites of action in disease, a clearer picture of the cells and their actio

Published 6 Months Ago
Guide to Quality Control for Viral Sequencing
Viral sequencing has evolved into a sophisticated tool, empowering researchers to unravel the intricacies of viral genomes and their implications on human health. As we delve into the realm of viral genomics, meticulous quality control (QC) steps become i

Published 7 Months Ago
Nanopore Sequencing 101: Choosing the Right Sequencing Device
Oxford Nanopore's technology empowers base modification analysis and nucleotide sequencing within a single read-length fragment, removing the necessity for conducting multiple sequencing experiments. This sets it apart from conventional methods as it obvi

Published 7 Months Ago
Comparison Between Prime Editing and CRISPR-Cas9
The gRNA is designed to complement specific target sequences in the genome to guide the Cas9 protein to the desired location. Once the Cas9 protein reaches the target site, it causes a double-strand break in the DNA helix, which initiates a cellular repai

Published 7 Months Ago
Overview of Nanopore Sequencing
Nanopore sequencing, a pivotal component of long-read sequencing technology, has emerged as a formidable and promising approach in the realm of genomics and molecular biology. Spearheaded by the Oxford Nanopore Company, this innovative technology bears th

Published 7 Months Ago
Introduction, Features, Workflow, and Applications of Degradome Sequencing:
MicroRNAs (miRNAs) are a class of small endogenous non-coding RNAs of about 22 nt in length, with hairpin structures as precursors, which are widely found in animal, plant and viral organisms.

Published 7 Months Ago
Detect Fusion Transcripts by RNA Sequencing in Screening Tumors
A fusion gene is a chimeric gene formed by the fusion of partial sequences of two genes, usually due to chromosomal translocations or deletions. These chimeric genes can form abnormal transcripts or proteins in subsequent biological processes, which can l

Published 7 Months Ago
Guide to Experimental Design: Importance and How to Design a Good Experiment
Experimental design plays a crucial role in various research areas, providing a systematic framework for conducting experiments and obtaining reliable and interpretable results. Experimental design is critical, especially when trying to avoid attrition bi

Published 8 Months Ago
Sequencing of Gut Microbiome – The Approach to Uncover Many Secrets Hidden Inside Our Gut
Our bodies have a mutually beneficial relationship with trillions of microbes currently living within the gastrointestinal tract. The microbiome is known simply as numerous species creating this population. Through improving digestion, enforcing metabolis

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