Articles By kiko

Published 1 Year Ago
Navigating Microbial Diversity: Choosing Between 16S rRNA and Metagenome Sequencing for Your Research
At the forefront of this exploration are two pivotal techniques: 16S rRNA sequencing and metagenome sequencing. Each method offers unique strengths and applications, enabling scientists to unravel the intricacies of microbial ecosystems. This article prov

Published 1 Year Ago
Decoding the Conversation in Host-Pathogen Dynamics
However, recent advancements in RNA-seq technologies have introduced innovative methods that delve deeper into the post-transcriptional networks governed by small RNAs and the associated RNA-binding proteins within pathogens. Among these advancements, dua

Published 1 Year Ago
Unveiling the Microbial World by Understanding the Diversity and Impact of Microorganisms
In fact, studies have shown that all organisms have evolved from a single prokaryote or more commonly known as the Last Universal Common Ancestor (LUCA). Today, it has been estimated that there are about one trillion species of microorganisms in the worl

Published 1 Year Ago
Mastering Microbial Cultivation by Navigating Aerobic and Anaerobic Growth Challenges
Microorganisms have different growth requirements. When culturing them in the laboratory, it is essential to take note of these conditions because these are needed to be replicated in order to successfully grow microbial cultures in the laboratory. As men

Published 1 Year Ago
Exploring How RNC-RNA Sequencing Illuminates Translational Dynamics and Molecular Mechanisms
When a ribosome is affixed to the polypeptide (protein) it is making, the ribosome-nascent chain complex (RNC) is the compilation of molecules that make up the ribosome. One of several methods can be used to halt the synthesis of the nascent polypeptide.

Published 1 Year Ago
Sequencing Depth, Coverage and Read Types for NGS
Sequencing depth is the ratio of the total number of bases (bp) obtained by sequencing to the genome size, which is one of the indicators to evaluate the sequencing volume. There is a positive correlation between sequencing depth and genome coverage, and

Published 1 Year Ago
How to Choose ANOVA?
Analysis of variance (ANOVA) is a powerful statistical method that allows researchers to compare the means of two or more groups and determine if they are significantly different. It is a popular tool for testing hypotheses in a variety of fields in biolo

Published 1 Year Ago
How to Submit Sequence Data to GenBank
In addition to raw sequence data, you can also submit computationally assembled sequences, genomes, functional genomics data, microarray data, clinical data, genome variations, and other data types, such as PacBio methylation data. Submission to SRA, GEO,

Published 1 Year Ago
Advancements in Sequencing Technologies: Transforming Pathogen Detection and Clinical Diagnostic
Despite a multitude of microorganisms known to inflict human infections, prevailing diagnostic techniques merely scratch the surface, leaving a vast array of pathogens unidentified. This persistent challenge is further exacerbated by the continuous emerge

Published 1 Year Ago
Hybridization Capture and PCR Amplification: Comparing Two Key Enrichment Strategies in NGS
Amplicon-based approaches may be preferable for their simplified workflow and smaller amounts of required DNA. However, hybridization-based strategies are less likely to miss mutations and also perform better with respect to sequencing complexity and unif

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